Correction: Investigating the Proton Donor in the NO Reductase from Paracoccus denitrificans
نویسندگان
چکیده
[This corrects the article DOI: 10.1371/journal.pone.0152745.].
منابع مشابه
The nitric oxide reductase of Paracoccus denitrificans.
The nitric oxide (NO) reductase activity of the cytoplasmic membrane of Paracoccus denitrificans can be solubilized in dodecyl maltoside with good retention of activity. The solubilized enzyme lacks NADH-dependent activity, but can be assayed with isoascorbate plus 2,3,5,6-tetramethylphenylene-1,4-diamine as electron donor and with horse heart cytochrome c as mediator. Reduction of NO was measu...
متن کاملHydroxylamine as an inhibitor and terminal acceptor in the respiratory chain of the bacterium Paracoccus denitrificans.
Three sites of inhibitory action of hydroxylamine were identified in the respiratory chain of anaerobically grown bacterium Paracoccus denitrificans. Terminal oxidases were blocked at concentrations of 10(-4) to 10(-3) mol.l-1, and the inhibitor competed with artificial donor of electrons N, N, N', N'-tetramethyl-l, 4-phenylenediamine. In the anaerobic part of the respiratory chain inhibition o...
متن کاملNitric oxide-dependent proton translocation in various denitrifiers.
Respiration of NO resulted in transient proton translocation in anaerobically grown cells of four physiologically diverse denitrifiers. Paracoccus denitrificans, Rhodopseudomonas sphaeroides subsp. denitrificans, "Achromobacter cycloclastes," and Rhizobium japonicum gave, respectively, H+/NO ratios of 3.65, 4.96, 1.94, and 1.12. Antimycin A completely inhibited NO-dependent proton translocation...
متن کاملThe cytochrome c oxidase from Paracoccus denitrificans does not change the metal center ligation upon reduction.
Cytochrome c oxidase catalyzes the reduction of oxygen to water. This process is accompanied by the vectorial transport of protons across the mitochondrial or bacterial membrane ("proton pumping"). The mechanism of proton pumping is still a matter of debate. Many proposed mechanisms require structural changes during the reaction cycle of cytochrome c oxidase. Therefore, the structure of the cyt...
متن کاملProton transfer in bacterial nitric oxide reductase.
The NOR (nitric oxide reductase) from Paracoccus denitrificans catalyses the two-electron reduction of NO to N(2)O (2NO+2H(+)+2e(-)-->N(2)O+H(2)O). The NOR is a divergent member of the superfamily of haem-copper oxidases, oxygen-reducing enzymes which couple the reduction of oxygen with translocation of protons across the membrane. In contrast, reduction of NO catalysed by NOR is non-electrogen...
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عنوان ژورنال:
دوره 11 شماره
صفحات -
تاریخ انتشار 2016